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Elutioplate

TLC Plate Reader

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TLC Plate Title


About

What is Elutioplate

Elutioplate is a web-app to analyze TLC (Thin-Layer Chromatography) plates, from images of them. Elutioplate is able to calculate RF (Retention Factor), intensity, color and more. The manual can be found here.

Who & Contact

Elutioplate is developed by Victor Kyllesbech at Vrije Universiteit, and has been published as [Publication Title] in [Journal] in collaberation with [], detailing its use amuong chemistry students. Elutioplate is open-source and published under the [LISENCE] with code freely available on Github and Zenodo, If you want to contact me for Elutioplate-specific inquiries, please email elutioplate@kyll.dk

How to Cite

If you use Elutioplate in a context requiring citation, please cite our publication [Publication name and link] (DOI) as follows:

[Formatted Citation Here]


Disclaimer

Elutioplate is provided as is, with all faults, Elutioplate provide no gaurentee of results, use of elutioplate is at your own risk



Example Plate

Intensity Example

If you want to try out Elutioplate, you can download the Example TLC Plate used in the Manual here. This TLC plate contains various concentrations of N-Benzyl Benzamide, as listed in the table below. Using the intensity calculation, a linear relationship between concentration and intensity can be found.

Spot Concentration (µMol)
1 0.02528
2 0.05055
3 0.7583
4 0.1011
5 0.1264

Color/RF Example

Another example TLC plate, is also available showing different colors, and retention factor values for different spots, this plate can be downloaded Here.


Thanks to

Adrian G. Rossebø

I'd like to extend a special thanks to Adrian G. Rossebø, who provided me with the idea for Elutioplate and, has been instrumental in the testing, and provided images of TLC plate for testing, including the example TLC plate used in the manual .


Code Used in This Project

This project is build using OpenCV.JS using document scanner code adapted from Tony's tutorial and the simple-blob-detector by Jan Paul Posoma The dual-slider UI utilized is from Here, and code to limit text-input adapted from Here

Lanes

Total Plate Height :

Main Section Height :

Spot Properties

X-Position

X-Position

Size

Annotation

Lane

Center Color

Corrected Center Color

Auto Spot Detector

Threshold Step : 5

Threshold : 25 - 220

Min Seperation : 10

Repeatability : 2

Min Spot Area : 200

Min Circulairty : 0

Min Innertia : 0

Min Convexity : 0

Intensity

Intensity

Threshold : 15

Advanced Options

Expansion : 7

Color Sample Distance : 21


Download Data

Spot ID Lane Annotation Retention Factor Radius Intensity
(+)
Size Inverted Intensity Color
(+)
Unadjusted Color Average Color Average Uadjected Color Unit

Download Figure

General Options

Save/Load Plot Theme

Which Blob To Edit

Preview Background Color


Use Correct Aspect Ratio

Line Width : 2

Padding : 25

Add TLC Image

Outline

Line Width : 2

Corner Radius : 10

Fill Color

Stroke Color

Baselines

Draw Accurate Placement

Deposit Line : 0.1

Solvent Front : 0.9

Line Width : 2

Stroke Color

Lanes

Draw Lines

Draw Accurate Placement

Line Width : 2

Stroke Color

Line Dash



Draw Text

Font Size : 8

Text Color

Text Offset : 0.05

ZigZag Offset : 0

Draw ZigZag Lines

ZigZag Line Width : 1

ZigZag Line Color

Blobs

Which Blob To Edit

Center Radius : 8



Use Blob Base Color

Use Blob Adjusted Color

Circle Color



Write Annotation

Write Retention Factor

Text Color

Angle : 0

RF Digits : 2

Offset : 2

Font Size : 10



Draw Size

Line Color

Line Width : 1

Line Dash